There are generally four stages of tissue healing: hemostasis, inflammation, proliferative/granulative, and maturation/remodeling. Specific timelines of injury healing vary depending on numerous factors, such as the type of tissue (inert versus contractile), severity of injury, age of patient, and pre-injury status. We will mainly be referring to muscles, tendons, fascia (surrounds muscles) and ligaments in this post.
Acute Versus Chronic- What Does it Mean?
First, we will discuss the difference between an acute and chronic injury.
- An acute injury is characterized by a specific time, place, and instance that occurred that caused the injury. Typically, an impact or a fall.
- Developing an ankle sprain from tripping whilst running where immediate pain was felt in the ankle is an example of an acute mechanism of injury.
- Developing an ankle sprain from tripping whilst running where immediate pain was felt in the ankle is an example of an acute mechanism of injury.
- A chronic injury, on the other hand, is characterized by developing slowly over time from repetitive micro-trauma or overuse. This can cause symptoms such as a dull ache, persistent discomfort, and aggravation of symptoms with certain repetitive activities
- Developing pain on the outside of the elbow from repeated mousing whilst working a desk job is an example of a chronic injury.
- Developing pain on the outside of the elbow from repeated mousing whilst working a desk job is an example of a chronic injury.
- An acute injury can become chronic typically if it surpasses 3-6 months of healing (this does not include complex fractures or surgeries).
- An acute-on-chronic injury can occur when someone has been developing a chronic/overuse injury and then has an acute mechanism/trauma in addition to their previously-developed issue.
- Developing a lumbar disc herniation in someone who experiences chronic, benign lower back pain is an example of an acute-on-chronic injury. This could happen when someone with back pain bends over to lift something too heavy, and suddenly feels a pop followed by a sharp pain in their back +/- numbness/burning/tingling in the leg(s).
- Another example of an acute-on-chronic injury would be someone with chronic knee pain (such as patellofemoral pain) who suddenly plants and twists their knee and develops an ACL tear.
Stages of Tissue Healing- Overview
Each phase is a continuous sequence and range where the extremes are completely distinct, but the parts in between blend into one another without any clear or abrupt dividing lines This is important to note as we dive into stage-defining characteristics. This is also important to note if you are applying these stages to your own injury; it can be hard to determine where exactly you are in terms of tissue healing.
With that being said, the four stages are named as follows:
- Hemostasis
- Inflammatory
- Proliferative or granulative
- Maturation or remodeling
- The body’s innate, life-saving process to stop bleeding and initiate tissue repair.
- Three basic steps in this are vasoconstriction (narrowing blood vessels in which the bleeding occurs), creating a platelet plug (cells temporarily plug the region), and lastly, coagulation (forming a blood clot to stop the bleeding by use of a mesh-like protein called fibrin).
- This is a relatively short stage: about 4-6 hours, but can depend on the tissue damaged.
- For example, more vascular tissues like muscle will bleed for a lot longer than tissues like ligaments (with not as much blood supply).
Stage 2: Inflammation
- The immune system’s response to an injury. This is a protective mechanism to isolate the issue from the rest of the body and begin the healing process.
- Chronic inflammation is different and can be rather dangerous. Again, we are focusing on acute injuries.
- This stage is the most prominent the first week after injury: 1-7 days
- Fundamental signs of the inflammatory period:
- Heat / calor
- Redness / rubor
- Swelling / tumor
- Pain / dolor
- Spasm (protective function)
- Loss of function / functio laesa
- Again, having this response to an injury is a helpful and protective mechanism, however we can still manage it and work to assist healing to the next stage.
- Therefore, we can guide and support the inflammation phase
- Vascular response: as mentioned, there is an initial vasoconstriction to stop bleeding, but this is followed by vasodilation (widening blood vessels), which allows capillaries to let fluid leak out which allows for vitamins and oxygenated blood to help with healing. This also helps clean up the damaged tissues and possible bacteria (such as in an open wound)
Treatment During the Inflammatory Phase
- Avoid painful activities whilst encouraging gentle range of motion
- Protect the region of injury
- Elevate to help with circulation
- Ice if sore/excess edema
Stage 3: Proliferation
- Once the injuring stimulus has ceased, haemostasis has been achieved, the inflammatory response is balanced and the wound is debris free, the proliferative stage of the healing cascade can begin to repair the defect.
- From day 3-20
- Here we want to cover the initial region on injury and begin to strengthen/repair the tissue
- There are four main components of this:
- Angiogenesis: the formation of new blood vessels to re-establish a healthy supply of nutrients and oxygen to the area of injury
- Fibroplasia: the development of fibroblasts (cells that make up connective tissue) migrating into the area of injury.
- Blood flow: as above
- Metabolism: cells converting energy (nutrients, oxygen) to use for tissue repair
Treatment During the Proliferative Phase
- Increase circulation
- Increase enzyme rate
- Promote collagen deposit (see next stage for more)
- Promote remodelling
- A great tool to support all of the above is physiotherapy for safe, guided soft tissue massage, manual therapy, exercises (mobility, strengthening, weight bearing), and sometimes, modalities such as therapeutic ultrasound for deep tissue.
Stage 4: Maturation / Remodelling
- From day 9 (overlapping with other phases) and onward (months to 2 years)!
- This phase results in the development of normal epithelium and maturation of the scar tissue. There is an important balance between both synthesis (creation) of new tissue as well as degradation of old/damaged tissue.
- In this stage, proteins such as collagen become very well organized and structured, rather than initially when the body is laying down various more disordered
Treatment During the Maturation/Remodelling Phase
- Here we want to influence alignment of collagen to maximize movement and minimize restrictions in order to prevent chronic, maladaptive inflammation
- A large emphasis on getting the injured region to mimic that of the uninjured side for unilateral injuries, or returning back to baseline level of function, sport, performance, daily activities in general
- This is the phase of physiotherapy where, in my experience, patients do not complete treatment. I find patients usually stop coming to physiotherapy once they feel 80-90% better, when in reality, that extra 10-20% is arguably the most important to not let their injury become chronic!
A Final Note…
In general, these stages of healing vary and have a lot of overlap. Physiotherapy can be extremely beneficial in determining which stage of healing you are in, how to manage and facilitate healing effectively, and how to progress safely!
References
Parsons, Joanne (2022). PT 6230 Applied Sciences for Physical Therapy: Part 2A. University of Manitoba. Microsoft PowerPoint.
Singh, Young, & McNaught (2017). The physiology of wound healing, Surgery (Oxford), Volume 35, Issue 9, Pages 473-477. https://doi.org/10.1016/j.mpsur.2017.06.004.
Soliman, A. M., & Barreda, D. R. (2023). Acute Inflammation in Tissue Healing. International Journal of Molecular Sciences, 24(1), 641. https://doi.org/10.3390/ijms24010641.




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